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Immunology and Infection
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可视化的活细菌细胞蛋白-DNA相互作用使用光活化的单分子跟踪
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
Please note that all translations are automatically generated.
Click here for the English version.
可视化的活细菌细胞蛋白-DNA相互作用使用光活化的单分子跟踪
DOI:
10.3791/51177-v
•
16:21 min
•
March 10, 2014
•
Stephan Uphoff
2
,
David J. Sherratt
,
Achillefs N. Kapanidis
1
Microbiology Unit, Department of Biochemistry
,
University of Oxford
,
2
Biological Physics Research Group, Clarendon Laboratory, Department of Physics
,
University of Oxford
Chapters
00:05
Title
01:32
Cell Slide Preparation
04:00
Preparing Microscopy Data Acquisition
06:43
Acquiring Photo-activated Localization Microscopy (PALM) Movies
08:16
PALM Movie Analysis
12:48
Representative Results: Pol1-DNA Binding in Single Bacterial Cells
15:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
光敏定位显微镜(PALM)结合单分子跟踪允许直接观察活的
大肠杆菌
细胞蛋白-DNA相互作用的定性和定量。
Tags
Protein-DNA Interactions
Live Bacterial Cells
Photoactivated Single-molecule Tracking
DNA Replication
Transcription
Repair
Chromosome Organization
DNA-binding Proteins
Photoactivated Localization Microscopy (PALM)
Single-molecule Tracking
DNA-binding Events
Protein Activity
Single-cell Level
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