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1Department of Bioengineering,Stanford University, 2Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research,Umeå University, 3Campus de Cantoblanco,Universidad Autonoma de Madrid, 4Department of Microbiology and Immunology,Stanford University School of Medicine
Chapters
- 00:05Title
- 01:43Day 1 – Lysing Bacterial Cultures
- 03:24Day 2 – Enzymatic Digestions
- 06:28Day 3 – Preparation of Samples for UPLC
- 09:29Results: UPLC Chromatograms of PG Digested from E. coli Sacculi
- 10:36Conclusion
A parede celular bacteriana é composta de peptidoglycan, uma rede macromolecular de fios de açúcar cruzados por peptídeos. A Cromatografia Líquida Ultra Performance fornece alta resolução e throughput para novas descobertas da composição peptidoglycan. Apresentamos um procedimento para o isolamento das paredes celulares (sacculi) e sua posterior preparação para análise via UPLC.
