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1Department of Biomedical and Molecular Sciences,Queen’s University, 2National Institute of Neurological Disorders and Stroke,Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory,National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition,The Hebrew University of Jerusalem
Chapters
- 00:05Title
- 01:14Growing Single Ice Crystals
- 03:18Determining Singularity and Orientation o the Ice Crystal
- 04:37Absorption of Fluorescently Labeled Antifreeze Proteins to a Single Ice Crystal
- 07:20Results: Fluorescence-based Ice Plane Affinity Analysis Images
- 08:18Conclusion
Proteínas anticongelantes (AFPs) se ligam a planos específicos de gelo para prevenir ou retardar o crescimento do gelo. A análise de afinidade de aviões de gelo baseada em fluorescência (FIPA) é uma modificação do método original de gravação de gelo para determinação de aviões de gelo ligados à AFP. Os AFPs são rotulados fluorescentemente, incorporados em cristais de gelo simples macroscópicos, e visualizados sob luz UV.
