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Visualization of G3BP Stress Granules Dynamics in Live Primary Cells
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Visualization of G3BP Stress Granules Dynamics in Live Primary Cells

Visualization of G3BP Stress Granules Dynamics in Live Primary Cells

DOI:
10.3791/51197-v

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10:12 min

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May 21, 2014

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,
1Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535

Chapters

  • 00:05Title
  • 01:22Culturing Mouse Embryonic Fibroblasts (MEFs)
  • 04:17Culturing Neurons
  • 06:22Visualizing G3BP-containing Stress Granule Assembly and Dynamics
  • 08:39Stress Granule Formation in MEFs and Murine Hippocampal Neurons
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

Stress granules (SGs) are cytoplasmic RNA granules containing stalled ribonucleoprotein particles (RNPs), and important in cellular response to various stresses. Dynamics of SGs can be followed in live cells by visualizing the localization of a tagged component of SGs in transfected primary cells after stress.

Tags

Keywords: G3BPStress GranulesLive Cell ImagingPrimary CellsMouse Embryonic FibroblastsNeuronsNeurodegenerative DiseasesGranule Dynamics

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