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Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes
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Bioengineering
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JoVE Journal Bioengineering
Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

DOI:
10.3791/51254-v

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12:24 min

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June 03, 2014

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, , , ,
1Department of Neuroscience and Cell Biology,University of Texas Medical Branch, 2Department of Biochemistry and Molecular Biology,University of Texas Medical Branch, 3Sealy Center for Structural Biology and Molecular Biophysics,University of Texas Medical Branch

Chapters

  • 00:05Title
  • 01:21Cryo-electron Microscopy of Factor VIII Bound to Lipid Nanotubes
  • 07:193D Reconstruction of Porcine Factor VIII Bound to Lipid Nanotubes
  • 08:21Electron Tomography of Porcine Factor VIII Bound to Lipid Nanotubes
  • 09:42Results: Factor VIII Helical Organization on Lipid Nanotubes
  • 11:42Conclusion

Summary

Automatic Translation

Automatic Translation

We present a combination of Cryo-electron microscopy, lipid nanotechnology, and structure analysis applied to resolve the membrane-bound structure of two highly homologous FVIII forms: human and porcine. The methodology developed in our laboratory to helically organize the two functional recombinant FVIII forms on negatively charged lipid nanotubes (LNT) is described.

Tags

Keywords: Cryo-electron MicroscopyFactor VIIIBlood CoagulationLipid NanotubesHemophiliaProtein StructureRecombinant FVIIIElectron Tomography3D Reconstruction

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