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JoVE Journal Neuroscience
Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
 

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Article DOI: 10.3791/51305-v • 08:27 min • April 16th, 2014
April 16th, 2014 •
1,2, 1,2, 3, 1,2,4, 1,2, 1,2
1Department of Veterans Affairs, Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition, University of Minnesota, 3Department of Integrative Biology and Physiology, University of Minnesota, 4Department of Medicine, University of Minnesota Medical School, University of Minnesota

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Summary
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Multiplex assays can provide beneficial information for basic cellular mechanisms and eliminate waste of reagents and unnecessary repetitive experiments. We describe here a multiplex caspase-3/7 activity assay, using fluorescent- and luminescent-based methods, to determine cell viability in an in vitro hypothalamic model following oxidative challenge with palmitic acid.

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Keywords: Caspase Multiplexing Apoptosis Hypothalamic Cell Model Palmitic Acid Resazurin Caspase-3/7 Luminogenic Substrate DEVD Saturated Fatty Acid Oxidative Stress
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