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A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
 

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Article DOI: 10.3791/51308-v • 07:59 min • March 25th, 2014
March 25th, 2014 •
1, 2, 3, 1
1Thayer School of Engineering, Dartmouth College, 2Institute for Immunology and Informatics, University of Rhode Island, 3Department of Computer Science, Dartmouth College

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Summary
Automatic Translation

Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design.  Here, a peptide-MHC II binding assay scaled to 384-well plates is described. This cost effective format should prove useful in the fields of protein deimmunization and vaccine design and development.

Tags

MHC II Binding Assay High-throughput 384-well Format Quantitative Analysis Peptide Epitope Immunogenic Epitope Protein Deimmunization Vaccine Design ELISA
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