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1Department of Pathology and Immunology, Faculty of Medicine,University of Geneva, 2Physics Department, GAP-Biophotonics,University of Geneva, 3Laboratoire d’Optique Biomédicale (LOB), Faculté des Sciences et Techniques de l’Ingénieur,École Polytechnique Fédérale de Lausanne, 4Department of Clinical Medicine, School of Medicine,Trinity College Dublin, 5School of Medicine and CRANN,Trinity College Dublin, 6Nikon AG Instruments
Chapters
- 00:05Title
- 02:26Human ESC Differentiation Protocol
- 03:50Air-liquid 3D Cultures of Beating Clusters, Labeling with SHG-NPs, and Non-linear Optical Imaging
- 05:52Results: Assessing Cardiac Activity of SHG-NP-labeled Cardiac Clusters
- 08:06Conclusion
Detalhes do protocolo estão previstas in vitro de células-tronco embrionárias humanas em matéria de rotulagem com a segunda geração de nanopartículas harmônicas. Também são apresentadas as metodologias de investigação hESC por microscopia multi-fotão e sua diferenciação em aglomerados cardíacas.
