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细胞标记和注射胚胎发展小鼠心脏
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Biology
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JoVE Journal Biology
Cell Labeling and Injection in Developing Embryonic Mouse Hearts
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细胞标记和注射胚胎发展小鼠心脏

DOI:
10.3791/51356-v

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07:20 min

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April 17, 2014

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, , , ,
1INSERM UMR-910,Aix-Marseille University, 2Skaggs School of Pharmacy and Pharmaceutical Sciences,University of California, San Diego

Chapters

  • 00:05Title
  • 01:49Collection of E9.5 and E10.5 Embryos for Ex vivo Injection
  • 03:10Ex vivo DNA or Cell Injection Under a Stereomicroscope
  • 04:35Results: Embryonic Heart Cell Labeling and Injection
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一系列的方法来注入染料，DNA载体，病毒和细胞，以监测来自于胚胎或多能干细胞内的小鼠胚胎在胚胎天（E）9.5和后期阶段内源性和移植细胞的两个细胞命运和表型的发展。

Tags

Cell LabelingCell InjectionEmbryonic Mouse HeartCell FateEmbryonic EnvironmentCell Lineage TracingMicroinjectionFluorescent DyesVirusShRNAStem Cell-derived Progenitor CellsOrgan DevelopmentEmbryo Culture

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