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Cellemærkning og Injektion i Udvikling Embryonale Mouse Hjerter
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JoVE Journal Biology
Cell Labeling and Injection in Developing Embryonic Mouse Hearts

Cellemærkning og Injektion i Udvikling Embryonale Mouse Hjerter

DOI:
10.3791/51356-v

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07:20 min

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April 17, 2014

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, , , ,
1INSERM UMR-910,Aix-Marseille University, 2Skaggs School of Pharmacy and Pharmaceutical Sciences,University of California, San Diego

Chapters

  • 00:05Title
  • 01:49Collection of E9.5 and E10.5 Embryos for Ex vivo Injection
  • 03:10Ex vivo DNA or Cell Injection Under a Stereomicroscope
  • 04:35Results: Embryonic Heart Cell Labeling and Injection
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en række metoder til at injicere farvestoffer, DNA-vektorer, virus og celler for at overvåge både celleskæbnen og fænotype af endogene og podede celler afledt fra embryonale eller pluripotente celler i musefostre på embryonisk dag (E) 9,5 og senere stadier udvikling.

Tags

Cell LabelingCell InjectionEmbryonic Mouse HeartCell FateEmbryonic EnvironmentCell Lineage TracingMicroinjectionFluorescent DyesVirusShRNAStem Cell-derived Progenitor CellsOrgan DevelopmentEmbryo Culture

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