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核苷三磷酸 - 从合成到生化特征
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JoVE Journal Chemistry
Nucleoside Triphosphates – From Synthesis to Biochemical Characterization
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核苷三磷酸 - 从合成到生化特征

DOI:
10.3791/51385-v

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15:22 min

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April 03, 2014

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, ,
1Department of Chemistry and Biochemistry,University of Bern

Chapters

  • 00:05Title
  • 02:30Synthesis of the Modified Nucleoside Triphosphates
  • 05:56HPLC Purification of the Modified Nucleoside Triphosphates
  • 07:40Primer Extension Reactions and TdT Polymerizations
  • 11:05Results: Biochemical Characterization of Nucleoside Triphosphates
  • 14:02Conclusion

Summary

Automatic Translation

Automatic Translation

本文所描述的协议旨在解释和删节的复杂路线导致修饰的三磷酸核苷的方法的许多障碍。因此，该协议有利于激活这些构建模块两者的合成及其可用性的实际应用。

Tags

Nucleoside TriphosphatesPhosphoramidite PrecursorsSolid-phase SynthesisModified Nucleic AcidsFunctional TaggingRibozymesDNAzymesPhosphorous(III)-based ReagentsPrimer Extension ReactionsTdT Tailing PolymerizationChemical Biology

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