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一个在体内交联方法分离蛋白复合物从果蝇胚胎
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JoVE Journal Biology
An in vivo Crosslinking Approach to Isolate Protein Complexes From Drosophila Embryos
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一个在体内交联方法分离蛋白复合物从果蝇胚胎

DOI:
10.3791/51387-v

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08:51 min

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April 23, 2014

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, , ,
1Department of Biological Sciences,Murray State University

Chapters

  • 00:05Title
  • 01:14Preparing Large Apple Juice Agar Plates
  • 02:04Collecting Embryos and Pre-crosslinking Treatment
  • 04:35In vivo Crosslinking
  • 05:34Preparing Proteins and Immunoprecipitation
  • 07:10Results: Crosslinked Tud Protein Complex Analysis
  • 08:23Conclusion

Summary

Automatic Translation

Automatic Translation

多组分蛋白复合物的细胞功能和发展过程中发挥关键作用。在这里，我们描述了用于从果蝇胚胎分离的天然蛋白质复合物后，随后交联的复合物用于随后的结构-功能分析的纯化在体内交联的方法。

Tags

Keywords: In Vivo CrosslinkingProtein ComplexesDrosophila EmbryosImmunopurificationTudor Protein ComplexGermline DevelopmentMass SpectrometryProtein PurificationMultisubunit Protein ComplexesNative EnvironmentCell LysisFormaldehydeMethylated ArgininesMethylated Lysines

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