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Journal
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Immunology and Infection
/
人中性粒细胞流动室粘附实验
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Human Neutrophil Flow Chamber Adhesion Assay
Please note that all translations are automatically generated.
Click here for the English version.
人中性粒细胞流动室粘附实验
DOI:
10.3791/51410-v
•
12:42 min
•
July 02, 2014
•
Yebin Zhou
,
Dennis F. Kucik
3,4
,
Alexander J. Szalai
,
Jeffrey C. Edberg
1
Genetics and Genomic Sciences Graduate Program
,
University of Alabama at Birmingham
,
2
Birmingham Veterans Affairs Medical Center
,
3
Department of Pathology
,
University of Alabama at Birmingham
,
4
Department of Biomedical Engineering
,
University of Alabama at Birmingham
,
5
Department of Medicine
,
University of Alabama at Birmingham
Chapters
00:05
Title
01:50
HUVEC Layer Preparation
03:07
Purified Ligand Coating
05:11
Neutrophil Separation
08:33
Flow Chamber Adhesion Assay
10:20
Results: The Flow Chamber Assay Enables Analyses of Adhesive Properties of Human Peripheral Blood Neutrophils to HUVECs and Purified Ligand Substrates
11:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
报道定量中性粒细胞粘附的方法。此方法创建类似于血管遇到了动态流环境。它使中性粒细胞粘附到任何纯化黏附分子(配体)或类似
体内
环境与剪切应力的上下文内皮细胞基板(HUVEC)的调查。
Tags
Neutrophil Adhesion
Endothelial Cells
Inflammation
β2 Integrin
ICAM
Autoimmune Disease
Flow Chamber
Shear Stress
HUVEC
Adhesion Receptors
Article
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