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标记的细胞核从亲和力为基础的隔离<em>果蝇</em>组织的基因表达分析

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JoVE Journal Biology

Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

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标记的细胞核从亲和力为基础的隔离<em>果蝇</em>组织的基因表达分析

DOI:

10.3791/51418-v

•

12:48 min

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March 25, 2014

•

Jingqun Ma, Vikki Marie Weake

¹Department of Biochemistry,Purdue University

Chapters

00:05Title
01:30Preparation of Antibody Coated Magnetic Beads and Extraction of Nuclei from Drosophila Larval Tissues
04:16Collection of Pre-isolation Samples
06:16Isolation of GFP-tagged Nuclei from Drosophila Larval Tissues
08:45Results: Analysis of Tagged-nuclei Enrichment “Pre-isolatoin” and “Post-isolation” Samples
12:28Conclusion

Summary

Automatic Translation

果蝇组织中往往含有细胞类型的非均相混合物。为了检验从一个特定的组织特异性细胞类型的基因表达，细胞核可遗传标记，随后使用具有亲和力为基础的方法分离出来。分离的细胞核，可用于下游应用，如基因表达分析和染色质免疫沉淀。

Tags

Affinity-based Isolation Tagged Nuclei Gene Expression Analysis Cell-specific Gene Expression Transcriptional Profiling Chromatin Immunoprecipitation Irregular Cellular Morphology Central Nervous System Laser Microdissection Fluorescence-activated Cell Sorting Affinity-based Isolation Of Tagged Nuclei Nuclear Envelope-localized EGFP Tag Gal4/UAS Binary Expression System Magnetic Beads

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