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Chapters
- 00:05Title
- 01:30Preparation of Antibody Coated Magnetic Beads and Extraction of Nuclei from Drosophila Larval Tissues
- 04:16Collection of Pre-isolation Samples
- 06:16Isolation of GFP-tagged Nuclei from Drosophila Larval Tissues
- 08:45Results: Analysis of Tagged-nuclei Enrichment “Pre-isolatoin” and “Post-isolation” Samples
- 12:28Conclusion
Drosophila væv ofte indeholder en heterogen blanding af celletyper. For at undersøge genekspression i specifikke celletyper fra et bestemt væv, kan kerner genetisk mærkede og efterfølgende isoleret ved anvendelse af en affinitet tilgang. Isolerede kerner kan anvendes til efterfølgende anvendelser, såsom genekspression analyse og kromatin immunofældning.
Tags
Affinity-based IsolationTagged NucleiGene Expression AnalysisCell-specific Gene ExpressionTranscriptional ProfilingChromatin ImmunoprecipitationIrregular Cellular MorphologyCentral Nervous SystemLaser MicrodissectionFluorescence-activated Cell SortingAffinity-based Isolation Of Tagged NucleiNuclear Envelope-localized EGFP TagGal4/UAS Binary Expression SystemMagnetic Beads
