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JoVE Journal Medicine
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
 

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Article DOI: 10.3791/51426-v • 10:26 min • March 11th, 2014
March 11th, 2014 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Advanced Cell Diagnostics, Inc.

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Summary
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The presence of high-risk HPV in head and neck tumor tissue is associated with favorable outcomes. The recently developed RNA in situ hybridization technique called RNAscope allows direct visualization of HPV E6/E7 mRNA in FFPE tissue sections.

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Keywords: RNAscope In Situ Detection Transcriptionally Active Human Papillomavirus Head And Neck Squamous Cell Carcinoma E6/E7 MRNA QRT-PCR Formalin-fixed Paraffin-embedded Tissue HPV16 HPV18 HPV31 HPV33 HPV35 HPV52 HPV58 Prognostic Biomarker Oropharyngeal Cancer
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