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Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
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Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage

Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage

DOI:
10.3791/51439-v

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09:41 min

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July 03, 2014

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, , , , , ,
1Multidisciplinary Neuroprotection Laboratories, Department of Anesthesiology,Duke University, 2Department of Neurology,Duke University, 3Department of Radiology,Duke University, 4Department of Neurobiology,Duke University

Chapters

  • 00:05Title
  • 01:58Preparations for Surgery
  • 02:54The Collagenase Injection Model
  • 04:54The Autologous Blood Injection Model
  • 06:48Post-operative Care
  • 07:25Hematoma Formation and Neurological Deficits of Injured Mice
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

Preclinical models of intracerebral hemorrhage are utilized to mimic certain aspects of clinical disease. Thus, mechanisms of injury and potential therapeutic strategies may be explored. In this protocol, two models of intracerebral hemorrhage are described, intrastriatal (basal ganglia) injections of autologous blood or collagenase.

Tags

Keywords: Intracerebral HemorrhageMurine ModelsIntrastriatal InjectionAutologous BloodClostridial CollagenaseCerebrovascular DiseaseNeuroinflammationCerebral EdemaNeurobehavioral OutcomesTherapeutic Interventions

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