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1School of Medical and Molecular Biosciences, Faculty of Science,University of Technology, Sydney, 2Centre for Health Technologies, Faculty of Engineering and Information Technology,University of Technology, Sydney, 3The Sydney Head and Neck Cancer Institute, Sydney Cancer Centre,Royal Prince Alfred Hospital
Chapters
- 00:05Title
- 01:50Small RNA Isolation from Serum
- 03:56RNA Resuspension into solution
- 04:38Results: Marked Improvements on the Traditional Chomczynski Method
- 06:08Conclusion
Questo protocollo descrive un metodo per l'estrazione di piccoli RNA da siero umano. Abbiamo usato questo metodo per isolare microRNA da siero cancro per uso in matrici di DNA e anche singleplex PCR quantitativa. Il protocollo utilizza fenolo e guanidina tiocianato reagenti con modifiche a produrre RNA di alta qualità.
