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结合单分子操纵和成像蛋白-DNA相互作用的研究
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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Please note that all translations are automatically generated.
Click here for the English version.
结合单分子操纵和成像蛋白-DNA相互作用的研究
DOI:
10.3791/51446-v
•
14:43 min
•
August 27, 2014
•
Carina Monico
2
,
Gionata Belcastro
,
Francesco Vanzi
3
,
Francesco S. Pavone
4,5,6
,
Marco Capitanio
4
1
LENS – European Laboratory for Non-linear Spectroscopy
,
University of Florence
,
2
Chemistry Research Laboratory
,
University of Oxford
,
3
Department of Biology
,
University of Florence
,
4
Department of Physics and Astronomy
,
University of Florence
,
5
National Institute of Optics-National Research Council, Italy
,
6
International Center of Computational Neurophotonics
Chapters
00:05
Title
02:39
Optical Tweezers Setup with Nanometer Stability
04:40
Microfluidics
08:36
Combined Optical Trapping and Single-molecule Fluorescence Imaging Experiments
11:50
Results: Detection of Single Protein Molecules Interacting with a Single DNA Molecule
12:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了仪器和方法,用于检测单荧光标记的蛋白质分子与悬浮两层光学俘获微球之间的单个DNA分子的相互作用。
Tags
Protein-DNA Interaction
Optical Tweezers
Single-molecule Fluorescence
Lactose Repressor
DNA Target Sequence
1D Diffusion
Molecular Motors
Single-molecule Manipulation
Single-molecule Imaging
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