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结合单分子操纵和成像蛋白-DNA相互作用的研究
JoVE Journal
Biology
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JoVE Journal Biology
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
DOI:

14:43 min

August 27, 2014

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Chapters

  • 00:05Title
  • 02:39Optical Tweezers Setup with Nanometer Stability
  • 04:40Microfluidics
  • 08:36Combined Optical Trapping and Single-molecule Fluorescence Imaging Experiments
  • 11:50Results: Detection of Single Protein Molecules Interacting with a Single DNA Molecule
  • 12:56Conclusion

Summary

Automatic Translation

在这里,我们描述了仪器和方法,用于检测单荧光标记的蛋白质分子与悬浮两层光学俘获微球之间的单个DNA分子的相互作用。

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