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Ved at kombinere Single-molekyle manipulation og Imaging for Studiet af protein-DNA interaktioner
JoVE Journal
Biology
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JoVE Journal Biology
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
DOI:

14:43 min

August 27, 2014

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Chapters

  • 00:05Title
  • 02:39Optical Tweezers Setup with Nanometer Stability
  • 04:40Microfluidics
  • 08:36Combined Optical Trapping and Single-molecule Fluorescence Imaging Experiments
  • 11:50Results: Detection of Single Protein Molecules Interacting with a Single DNA Molecule
  • 12:56Conclusion

Summary

Automatic Translation

Her beskriver vi instrumentering og metoder til påvisning af en enkelt fluorescens-mærkede proteinmolekyler, der interagerer med en enkelt DNA-molekyle udspændt mellem to optisk indespærrede mikrosfærer.

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