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A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

DOI:
10.3791/51470-v

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08:33 min

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December 15, 2014

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, , , ,
1Department of Medicine, Solna and CMM, Respiratory Medicine Unit,Karolinska Institutet, 2Safety Science, Global Regulator Affairs & Patient Safety,AstraZeneca Global Medicines Development

Chapters

  • 00:05Title
  • 01:13Generating an LPS Aerosol
  • 03:14Bronchoalveolar Lavage (BAG)
  • 05:12Formalin Fixation of Lung Tissue for Histological Assessment
  • 06:26Results: Representative Pulmonary Neutrophilia Data
  • 08:04Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for inducing neutrophilic pulmonary inflammation by challenge to aerosolized lipopolysaccharide by nebulization, to model acute lung injury. In addition, basic surgical techniques for lung isolation, tracheal intubation and bronchoalveolar lavage are also described.

Tags

Keywords: Pulmonary NeutrophiliaAerosolized LipopolysaccharideAcute Lung InjuryExperimental ModelPseudomonas AeruginosaBronchoalveolar LavageSterile Pulmonary Inflammation

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