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JoVE Journal
Immunology and Infection

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Selective Harvesting of Marginating-pulmonary Leukocytes
 

Selective Harvesting of Marginating-pulmonary Leukocytes

Article DOI: 10.3791/53849-v 07:06 min March 11th, 2016
March 11th, 2016

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Summary

Marginating-pulmonary leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe selective harvesting of this subpopulation of pulmonary leukocytes, by forced perfusion of the lungs in rats and mice. Marginating-pulmonary leukocytes seem critical in determining susceptibility to blood-borne and lung-related diseases.

Tags

Keywords: Selective Harvesting Marginating-pulmonary Leukocytes Lung Perfusion Rat Peristaltic Pump Butterfly Needles Hemostat Blood Collection Lung Perfusate
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