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Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

DOI:
10.3791/62937-v

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09:47 min

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August 13, 2021

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, , ,
1Department of Biology,San Diego State University, 2Institut de Biologie de l’École Normale Supérieure

Chapters

  • 00:04Introduction
  • 00:34Inducing Dauer Formation for Wild Nematodes on Nematode Growth Medium (NGM) Plates
  • 01:16Washing and Disinfecting the Nematodes
  • 03:12Removing the Antibiotic and SDS Solution
  • 04:08Propagate a Clean Nematode Strain
  • 05:27Intestinal Dissection and PCR Identification of Microbial Species
  • 08:17Results: The Host-microbe Interactions in the Context of the Intestines of Caenorhabditis tropicalis Strain JU1848
  • 09:16Conclusion

Summary

Automatic Translation

Automatic Translation

Wild Caenorhabditis nematodes are associated with many microbes, often in the gut lumen or infecting the intestine. This protocol details a method to enrich unculturable microbes colonizing the intestine, taking advantage of the resistance of the dauer cuticle.

Tags

Keywords: CaenorhabditisNematodesMicrobiomeEnrichmentIntestinal MicrobiomeUnculturable MicrobesOP51 Escherichia ColiDauer StageM9 Minimal Salts MediaTriton X-100AntibioticSDS Solution

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