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Assaying for Inorganic Polyphosphate in Bacteria
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assaying for Inorganic Polyphosphate in Bacteria

Assaying for Inorganic Polyphosphate in Bacteria

DOI:
10.3791/58818-v

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07:20 min

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January 21, 2019

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, , ,
1Department of Microbiology,University of Alabama at Birmingham, 2Division of Infectious Diseases,University of Alabama at Birmingham

Chapters

  • 00:04Title
  • 00:31Harvesting Bacterial Samples for Polyphosphate Extraction
  • 01:20Cell Lysates Protein Content Measurement
  • 02:09Polyphosphate Extraction
  • 03:17Polyphosphate Digestion
  • 04:09Free Phosphate Detection and Cellular Polyphosphate Content Calculation
  • 05:07Results: Production of polyP in Different Bacteria
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a simple method for rapid quantification of inorganic polyphosphate in diverse bacteria, including Gram-negative, Gram-positive, and mycobacterial species.

Tags

Keywords: Inorganic PolyphosphateBacterial Stress ResponsePolyP ExtractionPolyP QuantificationLactobacillus ReuteriGITC Lysis BufferBradford AssayEthanol PrecipitationSilica MembraneTris-HCl BufferPhosphate Standards

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