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Chapters
- 00:05Title
- 00:11Introduction
- 01:32Overview
- 01:37Competitive Elution with Biotin
- 02:44Denaturing Elution with SDS
- 03:26Chemical Cross-linking of the Resin
- 04:10Protocol
- 04:13Cross-linking of the Resin with BS3
- 07:22Quenching of the Cross-linking Reaction
- 09:50Binding of the Bait Protein and Associated Complexes to the Resin
- 12:18Washing of the Resin
- 14:23Elution of Specific Protein Complexes
- 15:32Representative Results
- 17:18Conclusion
