Journal
/
/
体外聚集测定使用tau蛋白的蛋白质
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
DOI:

09:22 min

January 02, 2015

, ,

Chapters

  • 00:05Title
  • 01:19Preparations
  • 02:14Terminal Assay with No Dye
  • 04:44Continual Mode Assay Using a Plate Reader With Dye
  • 06:14Continual Mode Assay Using a Compact Spectrofluorometer With Dye
  • 07:33Results: Tau Aggregation Assayed by Different Modes and Instruments
  • 08:34Conclusion

Summary

Automatic Translation

未修饰的和磷酸化的tau蛋白以两种体外聚集测定中使用以显示磷酸化依赖快速聚合动力学。这些测定铺路用于可调节tau蛋白的倾向,以形成背后阿尔茨海默氏病的进展纤丝化合物未来画面。

Related Videos

Read Article