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Nanomanipulation of Single RNA Molecules by Optical Tweezers
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Nanomanipulation of Single RNA Molecules by Optical Tweezers
DOI:

06:59 min

August 20, 2014

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Chapters

  • 00:05Title
  • 01:15Nanomanipulation of Single RNA Molecules
  • 04:53Results: Manipulating Single Hairpin Molecules
  • 06:19Conclusion

Summary

Automatic Translation

Optical tweezers have been used to study RNA folding by stretching individual molecules from their 5’ and 3’ ends. Here common procedures are described to synthesize RNA molecules for tweezing, calibration of the instrument, and methods to manipulate single molecules.

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