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Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
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Chapters
- 00:05Title
- 01:07Tweezer Setup
- 02:45Calibrating Apparent Size of the Bead to Axial Position
- 04:16Mapping the Optical Potential of the Manipulation Beam
- 05:10Stretching the DNA Sample
- 08:27Conclusion
We illustrate the use of a constant force axial optical tweezers to explore the mechanical properties of short DNA molecules. By stretching DNA axially, we minimize steric hindrances and artifacts arising in conventional lateral manipulation, allowing us to study DNA molecules as short as ~100 nm.
Tags
StretchingShort SequencesDNAConstant ForceAxial Optical TweezersSingle-molecule TechniquesExperimental DifficultiesBiological EventsHistone BindingProtein-mediated LoopingMechanical PropertiesPackaging Of DNACompact NucleosomesChromatin FibersPractical GuideOptical Tweezing TechniqueContour LengthsSubmicron Length DNABeadCoverslipAxial PositionSteric EffectsMicrospheres
