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伸展的DNA短序列恒力轴向光镊
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Bioengineering
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JoVE Journal Bioengineering
Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
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伸展的DNA短序列恒力轴向光镊

DOI:
10.3791/3405-v

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08:48 min

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October 13, 2011

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, ,
1LSA Biophysics,University of Michigan , 2LSA Biophysics, Department of Physics,University of Michigan

Chapters

  • 00:05Title
  • 01:07Tweezer Setup
  • 02:45Calibrating Apparent Size of the Bead to Axial Position
  • 04:16Mapping the Optical Potential of the Manipulation Beam
  • 05:10Stretching the DNA Sample
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

我们说明了使用一个恒定的力的轴向光镊探索短的DNA分子的力学性能。轴向拉伸DNA，我们尽量减少在传统的横向操作所产生的立体障碍和文物，使我们能够研究DNA分子的短〜100 nm之间。

Tags

StretchingShort SequencesDNAConstant ForceAxial Optical TweezersSingle-molecule TechniquesExperimental DifficultiesBiological EventsHistone BindingProtein-mediated LoopingMechanical PropertiesPackaging Of DNACompact NucleosomesChromatin FibersPractical GuideOptical Tweezing TechniqueContour LengthsSubmicron Length DNABeadCoverslipAxial PositionSteric EffectsMicrospheres

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