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Bioengineering

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JoVE Journal Bioengineering
Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy
 

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy

Article DOI: 10.3791/50950-v • 11:34 min • December 20th, 2013
December 20th, 2013 •
*1, *1, 1, 1
1Lehrstuhl für Angewandte Physik and Center for Nanoscience, Ludwig-Maximilians-Universität
* These authors contributed equally

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Summary
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Cellulosomes are multienzyme complexes designed for digesting cellulose. AFM-based SMFS was used to study the mechanical properties and folding configuration of cellulosome-associated protein assemblies. We present a complete workflow for protein immobilization, data acquisition, and data analysis to study the interactions of individual receptor-ligand complexes involved in cellulosome assembly.

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Keywords: Cellulosomes Receptor-ligand Systems Atomic Force Microscopy (AFM) Single-molecule Force Spectroscopy (SMFS) Cohesin-dockerin Interactions Binding Parameters Unfolding Forces Kinetic Off-rates Potential Widths
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