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A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

DOI:
10.3791/59360-v

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08:13 min

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April 08, 2019

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, , ,
1CREOL, The College of Optics and Photonics,University of Central Florida, 2Andor Technology,Oxford Instruments

Chapters

  • 00:04Title
  • 00:28Preliminary Setup
  • 01:11Setting up the Detection Path
  • 02:14Setting up the Excitation Path and the Cylindrical Lenses
  • 04:59Testing Tile Imaging
  • 05:53Highly Inclined Swept Tile (HIST) Imaging
  • 06:52Results: Highly Inclined Swept Tile Microscopy of Labeled DNA and RNA
  • 07:38Conclusion

Summary

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Automatic Translation

A detailed instruction is described on how to build a highly inclined swept tile (HIST) microscope and its usage for single-molecule imaging.

Tags

Keywords: Single-molecule ImagingExtended Field-of-viewSwept Tile MicroscopePiezo StageCollimated Light SourceDichroic MirrorTube LensMulti-band Pass FilterFold-mirrorObjective LensAiry Disc

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