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一种超越BRET步骤：通过荧光激发无限制自发光（FUEL）

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

Please note that all translations are automatically generated. Click here for the English version.

一种超越BRET步骤：通过荧光激发无限制自发光（FUEL）

DOI:

10.3791/51549-v

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07:04 min

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May 23, 2014

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Joseph Dragavon, Carolyn Sinow, Alexandra D. Holland, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson, Samantha Blazquez, Kelly L. Rogers, Régis Tournebize^6,7, Spencer L. Shorte

¹Plate-Forme d'Imagerie Dynamique, Imagopole,Institut Pasteur, ²Department of Radiation Oncology,Stanford School of Medicine, ³Service Hospitalier Frédéric Joliot,Institut d'Imagerie Biomédicale, ⁴Vanderbilt School of Medicine, ⁵The Walter & Eliza Hall Institute of Medical Research, ⁶Unité INSERM U786,Institut Pasteur, ⁷Unité de Pathogénie Microbienne Moléculaire,Institut Pasteur

Chapters

00:05Title
01:09Basic Recapitulation of FUEL
02:32FUEL Over Varying Distances
04:49Investigating Potential FUEL Pairs
05:53Results: Analysis of the Various FUEL Demonstrations
06:40Conclusion

Summary

Automatic Translation

扩大荧光通过调查的有关原则和展示与众多的荧光基团和抗体有针对性的条件，其兼容性的基础和适用性由非绑定从激发发光（燃料）。

Tags

FUEL Fluorescence Luminescence BRET Radiative Excitation Energy Transfer Fluorophores Fluorescent Nanoparticles Antibody-targeted FUEL In Vitro In Vivo Whole Animal Imaging

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