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荧光目标阵列的T细胞反应评价中的应用<em>在体内</em
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
DOI:

12:09 min

June 19, 2014

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Chapters

  • 00:05Title
  • 01:39Fluorescent Target Array Preparation: CTV and CFSE Labeling
  • 04:54FTA Preparation: Peptide Pulsing and Cell Washing
  • 07:50FTA Preparation: CPD Cell Labeling
  • 09:43FTA Assay in Response to VV/HIV Epitopes
  • 11:45Conclusion

Summary

Automatic Translation

监测体内详细的T细胞应答的能力,对于我们的免疫应答的理解的发展非常重要。在这里,我们描述了评估同时采用流式细胞仪> 250参数体内 T细胞检测使用荧光目标阵列(自由贸易协定)的。

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