This content is Free Access.
Chapters
- 00:05Title
- 01:25Preparation
- 01:53Sample Dissection
- 03:06UV Sperm Inactivation
- 03:58In Vitro Fertilization
- 05:36Observation and Handling of Haploid Embryos
- 06:09Results: Haploid and Diploid Embryos
- 07:04Conclusion
The zebrafish is a powerful model system for developmental biology and human disease research due to their genetic similarity with higher vertebrates. This protocol describes a methodology to create haploid zebrafish embryos that can be utilized for forward screen strategies to identify recessive mutations in genes essential for early embryogenesis.
