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Chapters
- 00:05Title
- 01:21Spheroplast Preparation
- 03:07Nanogold Uptake
- 03:44Fixation and Sectioning
- 04:42Silver Enhancement for Nanogold Particle Visualization
- 08:17Results: Yeast Endosomal Compartments are Visualized by Silver-enhanced Nanogold Labeling
- 09:09Conclusion
Gær, Saccharomyces cerevisiae, har været en vigtig model organisme for at identificere og studere gener, der regulerer den biogenese og funktioner endosomale system. Her præsenterer vi en detaljeret protokol for særlig mærkning af de endosomale rum til ultrastrukturelle studier.
