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Chapters
- 00:05Title
- 01:12Cell Culture
- 02:05Bead Aggregation
- 05:05Data Analysis
- 06:42Determining the Calcium-dependent Adhesive Ability of Zebrafish N-cadherin
- 07:53Conclusion
Here we present a simple, rapid method for characterizing the intrinsic adhesive properties of putative cell adhesion molecules. The secreted, epitope-tagged ectodomain of a cell adhesion molecule is captured from the culture medium on small, uniform functionalized beads. These beads can then be used immediately in simple bead aggregation assays.
