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JoVE Journal
Bioengineering

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Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
 

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Article DOI: 10.3791/51789-v 10:05 min August 7th, 2014
August 7th, 2014

Chapters

Summary

Several pathological biomarkers cannot be easily detected by current techniques because of their low concentration in biological fluids, the presence of degrading enzymes, and large amounts of high molecular weight proteins. Chemically functionalized hydrogel nanoparticles can harvest, preserve and concentrate low abundance proteins enabling the detection of previously undetectable biomarkers.

Tags

Hydrogel Nanoparticle Plasma Urine Low Abundance Proteins Biomarker Discovery Mass Spectrometry Immunoassay Poly(N-isopropylacrylamide) NIPAm Protein Capture Protein Concentration Detection Limit
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