A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 01:36Surgical Preparation
- 02:49Laparotomy and Ureter Division
- 03:59Right Nephrectomy
- 05:10Left Kidney Ischaemia and Reperfusion
- 08:06Representative Results: Injury
- 09:34Representative Results: Regeneration
- 11:30Conclusion
The mouse model of renal ischaemia reperfusion injury described here comprises of a right nephrectomy that provides control tissue and clamping of the left renal pedicle to induce ischaemia that results in acute kidney injury. This model uses a midline laparotomy approach with all steps performed via one incision.
