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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
 

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Article DOI: 10.3791/51845-v • 11:48 min • October 9th, 2014
October 9th, 2014 •
*1, *1, 1
1Division of Hematology/Oncology, Department of Medicine, Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine
* These authors contributed equally

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Summary
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Alternative splicing regulation has been shown to contribute to the epithelial-mesenchymal transition (EMT), an essential cellular program in various physiological and pathological processes. Here we describe a method utilizing an inducible EMT model for the detection of alternative splicing during EMT.

Tags

Alternative Splicing Epithelial-mesenchymal Transition EMT Twist E-cadherin N-cadherin Vimentin Isoform-specific Primers Quantitative RT-PCR Immunoblotting
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