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Chapters
- 00:05Title
- 02:05EMT (Epithelial-mesenchymal Transition) Induction
- 03:53Detection of Splice Isoforms Using qRT-PCR
- 06:34Examination of Splice Isoforms at the Protein Level
- 09:00Results: Alternative Splicing During Epithelial-mesenchymal Transition
- 10:32Conclusion
Alternative splicing regulation has been shown to contribute to the epithelial-mesenchymal transition (EMT), an essential cellular program in various physiological and pathological processes. Here we describe a method utilizing an inducible EMT model for the detection of alternative splicing during EMT.
