This content is Free Access.
Chapters
- 00:05Title
- 01:57Determination of Mutation Rate and Frequency
- 02:50Biotin Labeling
- 04:44Bead Labeling and Magnetic Sorting
- 07:25Determination of Replicative Age
- 09:09Results: Mutation Frequencies and Rates in Yeast
- 10:39Conclusion
Mutation rates in young Saccharomyces cerevisiae cells measured through fluctuation tests are used to predict mutation frequencies for mother cells of different replicative ages. Magnetic sorting and flow cytometry are then used to measure actual mutation frequencies and age of mother cells to identify any deviations from predicted mutation frequencies.
