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Chapters
- 00:05Title
- 02:35Plant Culture and Preparation
- 03:24Preparation of Experimental Materials, Solutions, and Radiotracers
- 05:22Direct Influx (DI) Measurement
- 07:54Compartmental Analysis by Tracer Efflux (CATE) Measurement
- 09:54Results: Radioactive Tracers Measure the Influx and Efflux of Nutrients and Toxicants in Plants
- 12:37Conclusion
In planta measurement of nutrient and toxicant fluxes is essential to the study of plant nutrition and toxicity. Here, we cover radiotracer protocols for influx and efflux determination in intact plant roots, using potassium (K+) and ammonia/ammonium (NH3/NH4+) fluxes as examples. Advantages and limitations of such techniques are discussed.
