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从2DE凝胶点在蛋白质功能：在课慢性淋巴细胞白血病学到HS1
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JoVE Journal Medicine
From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
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从2DE凝胶点在蛋白质功能：在课慢性淋巴细胞白血病学到HS1

DOI:
10.3791/51942-v

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10:18 min

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October 19, 2014

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, , , , , , ,
1Division of Molecular Oncology,IRCCS, San Raffaele Scientific Institute, 2Department of Haemato-Oncology,King’s College London, 3IFOM, FIRC Institute of Molecular Oncology, 4Università Vita-Salute San Raffaele

Chapters

  • 00:05Title
  • 01:52Human Tissue Samples and Cell Purification
  • 04:14Two-dimensional Electrophoresis
  • 06:38Cytoskeletal Activity Assays
  • 08:17Results: Identification of Prognostic Markers and Therapeutic Targets by 2DE and MS
  • 09:50Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一种协议，它把两个蛋白质组学技术，即2维电泳（2DE）和质谱（MS）来识别差异表达/翻译后修饰的蛋白质中的两个或多个基团主样品。这种做法，再加上功能性实验，允许预后标志物/治疗靶点的鉴定和表征。

Tags

2DE-gelProteomic ApproachChronic Lymphocytic LeukemiaDifferentially Expressed ProteinsMass SpectrometryProtein FunctionTumor InitiationTumor ProgressionLeukemic Cell Lysates2-dimensional ElectrophoresisPost-translational ModificationsMigrationAdhesionF-actin Polymerization

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