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Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.
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Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.

DOI:
10.3791/51967-v

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07:52 min

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February 08, 2015

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1Department of Hemostasis and Thrombosis,Leiden University Medical Center

Chapters

  • 00:05Title
  • 01:23Cell, Mouse, and Instrument Preparation
  • 02:34Orthotopic Injection
  • 04:19Organ Harvest
  • 05:39Results: Representative Othotopic and Metastatic Breast Tumor Growth in Mice
  • 07:25Conclusion

Summary

Automatic Translation

Automatic Translation

Cancer is a complex disease that is influenced by the tissue surrounding the tumor as well as local pro- and anti-inflammatory mediators. Therefore, orthotropic injection models, rather than subcutaneous models may be useful to study cancer progression in a manner that better mimics human pathology.

Tags

Breast CancerOrthotopic InjectionMammary Fat PadMouse ModelTumor GrowthGenetic ManipulationStromal CompartmentCancer Cell EngraftmentTumor AnalysisMetastasis

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