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Chapters
- 00:00Title
- 00:03Introduction
- 01:49Blood Collection
- 05:26Rat Serum Preparation
- 07:43Results
- 08:13Troubleshooting 1: Non-separation of Layers
- 08:39Troubleshooting 2: Mild Hemolysis
- 09:16Conclusion
Mammalian whole embryo culture (WEC) is widely used in teratology and developmental biology. Immediately centrifuged rat serum is commonly provided as a medium for both mouse and rat WEC. In this video, we demonstrate our standard protocol for the preparation of high-quality rat serum suitable for mammalian WEC.
