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Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
DOI:

11:25 min

October 15, 2014

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Chapters

  • 00:05Title
  • 02:35Preparation of a Blood Culture Bacterial Pellet by Ammonium Chloride Erythrocyte-lysing Procedure
  • 04:14Identification by MALDI-TOF MS
  • 05:54Bacterial Identification and Antibiotic Susceptibility Testing with an Automated Microbial System
  • 06:56Antibiotic Susceptibility Testing by Disk Diffusion Assay as Described by the European Committee on Antimicrobial Susceptibility Testing
  • 08:38Automated PCR-based Diagnostic Test for the Detection of Methicillin-resistant Staphyloccocus aureus (MRSA)
  • 09:49Results: Analysis of Blood Culture Pellet Quality and Downstream Applications
  • 10:55Conclusion

Summary

Automatic Translation

A rapid bacterial pellet preparation from a positive blood culture can be used as a sample for applications such as identification by MALDI-TOF, Gram staining, antibiotic susceptibility testing and PCR-based test. The results can be rapidly communicated to clinicians to improve the outcome of patients suffering from bloodstream infections.

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