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单一平面照明模块和一个宽场显微镜微毛细管法
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Engineering
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JoVE Journal Engineering
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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单一平面照明模块和一个宽场显微镜微毛细管法

DOI:
10.3791/51993-v

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08:53 min

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August 15, 2014

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, ,
1Institute of Applied Research,Aalen University

Chapters

  • 00:05Title
  • 02:03Cell Spheroids Incubated with a Chemotherapeutic Drug
  • 02:55Light Sheet Adjustment
  • 04:09Cell Spheroid Application and Microscope Feed Synchronization
  • 05:31Measurement in Dynamic Liquid Environment and Data Acquisition and Analysis
  • 07:00Results: Imaging of Cell Spheroids Incubated with the Cytostatic Drug Doxorubicin
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

为单一平面照明显微镜的模块（SPIM）中描述了很容易适应的倒宽视场显微镜和3维细胞培养物进行了优化。样品位于一个矩形的毛细管，并通过微流体系统的荧光染料，药物制剂或药物可以以小批量应用。

Tags

Single Plane IlluminationLight Sheet MicroscopySPIM3D Cell CultureMulti-cellular Tumor SpheroidsMicro-capillaryWide-field MicroscopeFluorescence DetectionMicrofluidicsDoxorubicinGlutathione SensorCell Necrosis

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