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Epizomlar ve HDAC inhibitörleri kullanarak Blood Verimli iPS Hücre Üretimi
JoVE Journal
Biology
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JoVE Journal Biology
Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
DOI:

08:14 min

October 28, 2014

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Chapters

  • 00:05Title
  • 01:23Ficoll Separation of PBMCs and Expansion of Erythroblasts Days 0-6
  • 03:17Reprogramming Cells by Nulceofection Day 9
  • 04:51Plating Feeder Cells Day 10-11
  • 05:47Plating Cells on Feeder Cells and Changing Medium Day 12
  • 06:41Results: Representative Nucleofected and Inducible Pluripotent Stem (iPS) Cells
  • 07:46Conclusion

Summary

Automatic Translation

Burada bir episomuna göre yeniden programlama strateji ve histon deasetilaz inhibitörleri kullanılarak periferal kandan alınan insan uyarılmış pluripotent kök hücrelerin üretilmesi için bir protokol açıklar.

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