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Chapters
- 00:05Title
- 01:30Growth Kinetics in Liquid Culture Under Selective Conditions (GiLS)
- 03:36Calculation of the Minimal Doubling Time (MDT) Using MDTcalc
- 05:04Results: Representative Protein Degradation Analysis
- 06:45Conclusion
Here we describe a robust biological assay for quantifying the relative rate of proteolysis by the ubiquitin-proteasome system. The assay readout is yeast growth rate in liquid culture, which is dependent on the cellular levels of a reporter protein comprising a degradation signal fused to an essential metabolic marker.
