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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
 

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Article DOI: 10.3791/52022-v • 11:40 min • October 20th, 2014
October 20th, 2014 •
1,2, 3, 3, 1,2,3
1Harper Cancer Research Institute, 2Microbiology and Immunology, Indiana University School of Medicine, 3Department of Biological Sciences, University of Notre Dame

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Summary
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Manipulating temporal gene expression in differentiating embryonic stem cells (ESCs) can be achieved using inducible gene systems. However, generation of these cell lines is costly and time consuming. This protocol achieves rapid expression of a transgene in differentiating ES-derived cells and subsequent analysis of downstream hematopoietic differentiation.

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Keywords: Embryonic Stem Cells Hematopoietic Progenitor Cells Embryoid Bodies Retroviral Infection Gene Expression Inducible Transgene System Flow Cytometry MicroRNA Mesoderm Hematopoietic Differentiation
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